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目的探讨CyclinD1及其mRNA在皮肤病理性瘢痕及瘢痕癌中的表达及意义。方法激光扫描共聚焦显微技术,对病理性瘢痕和瘢痕癌组织进行CyclinD1免疫荧光双标记。采用免疫组织化学(SP)法分别检测正常皮肤组织、病理性瘢痕和瘢痕癌三组组织中CyclinD1蛋白的表达。采用核酸分子原位杂交技术检测三组组织中CyclinD1 mRNA的表达。结合图像分析,分别计算被检组织中所检各项指标的平均光密度和阳性面积,所有数据运用SPSS16.0软件包进行统计学分析。结果 CyclinD1在病理性瘢痕上皮中呈现较弱荧光,为弱阳性表达;在瘢痕癌组织中呈现较强荧光,为强阳性表达。CyclinD1蛋白、CyclinD1 mRNA在正常皮肤表皮呈阴性表达,在病理性瘢痕上皮中呈弱阳性表达,在瘢痕癌组织中呈强阳性表达。瘢痕癌组的表达(即阳性面积和平均光密度)与正常皮肤组及病理性瘢痕组相比较,差异均有统计学意义(P均<0.01);但正常皮肤组与病理性瘢痕组相比较,差异无统计学意义(P>0.05)。在瘢痕癌组织中,CyclinD1与CyclinD1 mRNA的表达呈正相关。结论 CyclinD1蛋白及其mRNA在病理性瘢痕上皮中的表达无特殊意义,其与瘢痕癌的发生有相关性。
Objective To investigate the expression and significance of CyclinD1 and its mRNA in skin pathological scar and scar cancer. Methods Laser scanning confocal microscopy was used to detect CyclinD1 double immunofluorescence in pathological scar and scar tissue. Immunohistochemistry (SP) was used to detect the expression of CyclinD1 protein in normal skin tissue, pathological scar and scar tissue. The expression of CyclinD1 mRNA in three groups was detected by in situ hybridization. Combined with image analysis, the average optical density and the positive area of each index in the seized tissue were calculated, and all the data were analyzed by SPSS16.0 software package. Results CyclinD1 showed weak fluorescence in the pathological scar epithelium, which was weakly positive expression. It showed strong fluorescence in the scar tissue and strong positive expression. CyclinD1 protein and CyclinD1 mRNA were negatively expressed in normal skin epidermis, weakly positive in pathological scar epithelium and strongly positive in scar tissue. Compared with normal skin group and pathological scar group, the expression (ie, positive area and average optical density) of scar tissue was significantly different (all P <0.01); however, the difference between normal skin group and pathological scar group , The difference was not statistically significant (P> 0.05). In the scar tissue, CyclinD1 and CyclinD1 mRNA expression was positively correlated. Conclusion The expression of CyclinD1 protein and its mRNA in pathological scar epithelium is of no special significance, which is correlated with the occurrence of scar cancer.