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采用荧光显微法观察处理后的甘蓝自花授粉的雌蕊柱头 ,发现甘蓝自交花粉粒在柱头上的萌发情况区别较大 ,自交不亲和系只有少数花粉粒萌发 ,且花粉管形态不正常 ,不能穿透乳突细胞壁 ,花粉管条数只有 0~ 8条伸长进入花柱 ;自交亲和系几乎都萌发长出正常的花粉管进入花柱 ,其条数在 18条以上 ,并能到达子房
Fluorescence microscopy was used to observe the stigma of self-pollination of self-pollinated cabbage. The results showed that the germination of self-pollinated cabbage in the stigma was different. Only a few pollen grains germinated in self-incompatible lines, and the pollen tube morphology was not Normal, can not penetrate the papillary cell wall, the number of pollen tube elongation of only 0 to 8 into the style; almost all of the self-compatibility of germination and growth of normal pollen tube into the style, the number of more than 18, and can Reach the ovary