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目的:探讨在体外受精胚胎-移植(IVF-ET)和冻融胚胎移植(FET)过程中,患者子宫内膜受体表达及内膜组织形态学对胚胎着床前期子宫内膜容受性的影响。方法:选择接受助孕的不孕症患者80例为研究对象,于尿LH阳性的6～10天刮取子宫内膜组织,分别进行HE染色和免疫组织化学链霉素抗生物素蛋白-过氧化酶连接法(S-P)对子宫内膜雌激素受体(ER)、孕激素受体(PR)及表皮上皮生长因子受体(EGFR)染色强度和染色分布进行半定量分析。随访IVF-ET、FET结局,分为IVF-ET妊娠组18例,IVF-ET非妊娠组26例,FET妊娠组14例,FET非妊娠组22例。结果:IVF-ET妊娠组和FET妊娠组的ER、PR、EGFR均比IVF-ET非妊娠组、FET非妊娠组积分值高(P<0.05)。IVF-ET妊娠组、FET妊娠组的分泌期比率比IVF-ET非妊娠组、FET非妊娠组高,但各组间差异无统计学意义(P>0.05)。结论:子宫内膜ER、PR、EG-FR表达影响胚胎着床前期子宫内膜容受性,影响胚胎移植结局,可作为预测妊娠结局的指标。
OBJECTIVE: To investigate the expression of endometrial receptors and endometrial morphology on endometrial receptivity in preimplantation embryos during in vitro fertilization embryo transfer (IVF-ET) and frozen-thawed embryo transfer (FET) influences. Methods: Eighty infertility patients who were selected to be assisted in pregnancy were selected as research objects. Endometrial tissue was scored 6 to 10 days after urinary LH was positive, HE staining and immunohistochemical streptomycin avidin Oxidase-linked immunosorbent assay (SP) was used to semi-quantitatively analyze the staining intensity and staining distribution of endometrial estrogen receptor (ER), progesterone receptor (PR) and epidermal growth factor receptor (EGFR) Followed up IVF-ET and FET outcomes were divided into IVF-ET pregnancy group 18 cases, IVF-ET non-pregnancy group 26 cases, FET pregnancy group 14 cases, FET non-pregnancy group 22 cases. Results: The ER, PR and EGFR in IVF-ET pregnancy group and FET pregnancy group were higher than IVF-ET non-pregnancy group and FET non-pregnancy group (P <0.05). The rate of secretory phase in IVF-ET pregnancy group and FET pregnancy group was higher than that in IVF-ET non-pregnancy group and FET non-pregnancy group, but there was no significant difference among the groups (P> 0.05). Conclusion: The expressions of ER, PR and EG-FR in endometrium affect the endometrial receptivity of embryo implantation and affect the outcome of embryo transfer, which may be used as an index to predict pregnancy outcome.