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通过离子注入技术对枯草芽孢杆菌生防菌Bs-916突变选育,得到一株拮抗性能比出发菌Bs-916提高15%以上的突变菌株H-74。通过对其抗菌物质的高效液相色谱(HPLC)分析表明,H-74分泌的脂肽类抗菌物质主要是表面活性素。在PDA平板上的水稻纹枯病菌菌丝抑制试验中,它能有效抑制新生菌丝的生长,并使细胞内含物外泄。盆栽控病试验表明,它对水稻纹枯病菌的相对防效达到73.35%。将H-74分泌的脂肽类抗菌物质单喷于水稻植株上,测定与植物抗病相关的超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)活性的变化,发现其对植物抗病相关酶活性表达有明显的诱导作用。结果还显示同时接种脂肽类抗菌物质和水稻纹枯病菌处理对3个酶活性影响最大。
The Bs-916 mutant of B. subtilis was selected by iontophoresis to obtain a mutant strain H-74 with a higher antagonistic activity than the original strain Bs-916 by more than 15%. High performance liquid chromatography (HPLC) analysis of antibacterial substances showed that the lipopeptide antibacterial substance secreted by H-74 is mainly surface active. In the mycoplasma sheath inhibition test on R. solanacearum on PDA plates, it effectively inhibits the growth of new mycelia and exudes cell contents. Pot disease control test showed that its relative control effect on Rhizoctonia solani reached 73.35%. The lipopeptide antibacterial substances secreted by H-74 were sprayed on rice plants to determine the activities of superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO) The results showed that it had a significant induction effect on the expression of enzyme activity related to disease resistance in plants. The results also showed that simultaneous inoculation of lipopeptide antibacterial substances and Rhizoctonia solani treatment had the greatest effect on the three enzyme activities.