论文部分内容阅读
目的:观察磁性四氧化三铁(Fe3O4)纳米粒子对肝癌细胞的体外作用,并研究外加稳恒磁场(SMF)或交变磁场(EMF)对Fe3O4纳米粒子作用的影响。方法:光镜下观察CBRH-7919细胞对Fe3O4纳米粒子的吞噬作用;MTT法检测Fe3O4纳米粒子对大鼠肝癌细胞株CBRH-7919的毒性及外加磁场的影响;流式细胞术检测外加磁场作用下Fe3O4纳米粒子对细胞凋亡及线粒体膜电位的影响。结果:光镜下可见CBRH-7919细胞吞噬大量Fe3O4纳米粒子入胞浆,且交变磁场作用下细胞的吞噬量增加。30～100μg/mL Fe3O4纳米粒子作用于CBRH-7919细胞未产生细胞毒性,稳恒磁场对其作用无影响,而交变磁场能增加Fe3O4纳米粒子的毒性,使细胞活性降低、凋亡率增加、线粒体膜电位降低。结论:交变磁场能增加CBRH-7919细胞对Fe3O4纳米粒子的吞噬并产生细胞毒性。
OBJECTIVE: To observe the effect of magnetic Fe3O4 nanoparticles on hepatocarcinoma cells in vitro and to study the effect of applied magnetic field (SMF) or alternating magnetic field (EMF) on the effect of Fe3O4 nanoparticles. Methods: The phagocytosis of Fe3O4 nanoparticles by CBRH-7919 cells was observed by light microscopy. The effects of Fe3O4 nanoparticles on the toxicity and magnetic field of rat hepatoma cell line CBRH-7919 were detected by MTT assay. Flow cytometry Effect of Fe3O4 Nanoparticles on Apoptosis and Mitochondrial Membrane Potential. Results: Under the light microscope, CBRH-7919 cells phagocytosed a large amount of Fe3O4 nanoparticles into the cytoplasm, and the phagocytosis of cells under the alternating magnetic field increased. The effect of the magnetic field of 30 ~ 100μg / mL Fe3O4 nanoparticles on CBRH-7919 cells did not produce cytotoxicity, while the steady magnetic field had no effect on the effect of the magnetic field. However, the alternating magnetic field could increase the toxicity of Fe3O4 nanoparticles and decrease the cell viability and apoptosis rate. Mitochondrial membrane potential decreases. Conclusion: Alternating magnetic field can increase the phagocytosis of Fe3O4 nanoparticles in CBRH-7919 cells and produce cytotoxicity.