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目的:建立高效液相色谱法测定复方骨宁酊中槲皮素含量的方法。方法:大连依利特C18(4.6 mm×250 mm,5μm),流动相为甲醇-水-冰醋酸(50∶50∶1.5),流速为1.0mL/min,检测波长为373nm,柱温25℃。结果槲皮素浓度在0.095～1.520μg范围内呈良好线性关系(R=0.999970,n=6),加样回收率在96.94%～100.01%范围内,RSD≤0.94%(n=9)。结论:本方法操作简便、快速、准确,可用于该制剂的质量控制。
Objective: To establish a method for the determination of quercetin in Fufang Guining tincture by high performance liquid chromatography. METHODS: Dalian elite C18 (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was methanol-water-glacial acetic acid (50:50:1.5) at a flow rate of 1.0 mL / min. The detection wavelength was 373 nm and the column temperature was 25 ℃. Results There was a good linear relationship between the concentration of quercetin and 0.095 ~ 1.520μg (R = 0.999970, n = 6). The recoveries were within the range of 96.94% ~ 100.01% with RSD≤0.94% (n = 9). Conclusion: The method is simple, rapid and accurate and can be used for the quality control of this preparation.