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采用放射性配基结合分析法,以[~2H]-哌唑嗪和[~3H]-双氢烯丙洛尔测定大鼠肺脏α_1-和β-肾上腺素能受体(α_1-AR和β-AR)。根据Scatchard公式计算受体的最大结合容量(Bmax)和平衡解离常数(K_D)。结果表明,大鼠肺脏含高密度α_1-AR和β-AR。它们具有可饱和性、可逆性、高亲和力和立体专一性。 大鼠静注大肠杆菌内毒素诱导急性肺损伤后,α_1-AR和β-AR的Bmax值分别降低35％和43％。K_D值无变化。该组静注伊文思蓝判定肺微血管通透性指数(PMPI),显著高于对照组和山莨菪碱等组(P<
The levels of α 1 - and β - adrenergic receptors (α 1 - AR and β - adrenergic receptors) in rat lung were measured by radiolabeled ligand binding assay [~ 2H] - prazosin and [~ 3H] AR). The maximum binding capacity (Bmax) and equilibrium dissociation constant (K_D) of the receptor were calculated according to the Scatchard formula. The results showed that the rat lungs contained high density α_1-AR and β-AR. They have saturability, reversibility, high affinity and stereospecificity. After acute lung injury induced by E. coli lipopolysaccharide (LPS) in rats, the Bmax values of α 1 -AR and β-AR decreased by 35% and 43%, respectively. K_D value no change. The group intravenous Evans blue determination of pulmonary microvascular permeability index (PMPI), was significantly higher than the control group and anisodamine group (P <