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目的 探讨肝病患者抗氧化能力及一氧化氮合酶 (NOS)表达的临床价值。方法 收集 2 6例急性肝炎(AH )、36例慢性肝炎 (CH)、13例肝炎后肝硬化 (LC)和 2 3例原发性肝癌 (HCC)患者的血清 ,分别检测其总抗氧化能力(TAO)、NOS浓度及一氧化氮 (NO)水平 ,分析它们在肝脏疾病中的改变机制。结果 患者血清TAO在AH、CH中异常率为 80 % ,在LC和HCC为 5 0 % ;血清中NOS活性在肝病患者中的异常率在 70 %左右 ;NO水平在AH、CH和LC中异常率为 70 % ,HCC组为 48%。AH组、CH组TAO平均水平明显高于对照组 ,但LC和HCC组的差异不明显 ;肝病患者NO和NOS平均浓度均显著高于对照组 ,但HCC患者NOS和NO平均浓度均低于AH、CH和LC组患者。结论 肝病患者血清NOS水平与NO浓度呈高度正相关 ,NO增加可能对肝细胞起保护作用。
Objective To investigate the clinical value of antioxidant capacity and nitric oxide synthase (NOS) expression in patients with liver disease. Methods Serum levels of 26 patients with acute hepatitis (AH), 36 patients with chronic hepatitis (CH), 13 patients with posthepatitic cirrhosis (LC) and 23 patients with primary liver cancer (HCC) were collected and their total antioxidant capacity (TAO), the concentration of NOS and the level of nitric oxide (NO) were measured and their changes in liver diseases were analyzed. Results The abnormal rate of serum TAO in AH, CH was 80%, and in LC and HCC was 50%. The abnormal rate of serum NOS activity was about 70% in patients with liver disease. The levels of NO were abnormal in AH, CH and LC The rate was 70% in the HCC group and 48% in the HCC group. The average level of TAO in AH group and CH group was significantly higher than that in control group, but the difference between LC and HCC group was not obvious. The average concentration of NO and NOS in liver disease group was significantly higher than that in control group. However, the average concentration of NOS and NO in HCC group were lower than AH , CH and LC patients. Conclusion Serum NOS level in patients with liver disease is highly correlated with NO concentration. Increased NO may play a protective role on hepatocytes.