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目的探讨黄芪百合颗粒对高原低氧模型小鼠脑组织氧化应激损伤的影响。方法将60只SPF级昆明小鼠随机分为对照组、模型组、黄芪百合颗粒低、中、高(1.75、3.5、7 mg·kg~(-1))剂量组。常规饲养3 d后,灌胃给药,每日1次,连续17 d。从第14日起每日灌胃30 min后除对照组外其余各组小鼠于低氧舱中模拟高海拔低压低氧环境进行暴露,持续暴露3 d,眼球采血后处死小鼠。采用比色分析法测定脑组织中超氧化物歧化酶活性、丙二醛含量及总抗氧化能力,HE染色在显微镜下观察脑组织的形态变化进行病理评分并计数炎细胞数量。结果与对照组比较,模型组小鼠脑质量明显增加,超氧化物歧化酶活性和总抗氧化能力明显降低,脑丙二醛含量、脑组织病理评分和炎细胞数量均明显增加(P<0.01)。与模型组比较,黄芪百合颗粒中、高剂量组小鼠的脑组织超氧化物歧化酶活性和总抗氧化能力均升高,丙二醛含量、病理评分及炎细胞数均降低(P<0.05或P<0.01)。结论黄芪百合颗粒对高原低压低氧环境下小鼠脑损伤具有良好保护作用,可能是通过增强脑组织自由基清除能力实现的。
Objective To investigate the effects of Astragalus and Lily Granules on the oxidative stress injury in brain of hypoxia model rats. Methods Sixty SPF Kunming mice were randomly divided into control group, model group and astragalus membranaceus granules with low, medium and high doses (1.75, 3.5 and 7 mg · kg -1). Routine feeding 3 d, gavage, 1 day, for 17 days. From the 14th day after intragastric administration for 30 min, the mice in the other groups except the control group were exposed to simulated high altitude and hypoxia in the hypoxic chamber for 3 days. The mice were sacrificed after eyeball blood collection. The activity of superoxide dismutase (SOD), malondialdehyde (MDA) and total antioxidant capacity in brain tissue were measured by colorimetric assay. The morphological changes of brain tissue were observed with HE staining and the number of inflammatory cells was counted. Results Compared with the control group, the brain mass of the model group increased significantly, the activity of superoxide dismutase and the total antioxidant capacity decreased significantly, and the contents of MDA, brain tissue pathological score and number of inflammatory cells increased significantly (P <0.01 ). Compared with the model group, the activities of superoxide dismutase (SOD) and total antioxidant capacity (MDA), malondialdehyde (MDA), pathological score and the number of inflammatory cells in the medium and high dose groups of astragalus and Lily granules decreased Or P <0.01). Conclusion Astragalus and Lily Granules have a good protective effect on brain injury in mice exposed to plateau hypoxia and hypoxia, probably by enhancing the free radical scavenging ability of brain tissue.