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用淀粉凝胶电泳对中国 3个不同地理区域的小菜蛾种群和台湾敏感品系的抗性水平及酯酶同工酶进行了研究 ,结果发现 :生物测定显示 3个小菜蛾种群对杀虫双均没有明显的抗性 ;对阿维菌素的抗性分别为 1.2 1倍、4 .51倍和 1.2 5倍。在 Estα和 Estβ基因位点 ,北京种群中存在 11种同工酶 ,其中 4种为 α同工酶 ,7种 β同工酶 ;河北种群中存在 3种 α同工酶 ,5种 β同工酶 ;云南种群中存在 3种 α同工酶 ,3种 β同工酶。 3个种群酯酶基因多态性的产生可能是携带不同抗性酯酶基因的小菜蛾被动运输的结果 ;杀虫双对于能引起酯酶活性升高的杀虫药剂可能无交互抗性。
Starch gel electrophoresis was used to study the resistance level and esterase isozymes of diamondback moth, Plutella xylostella and three susceptible strains in three geographic regions of China. The results showed that bioassay showed that the resistance of three diamondback moth No obvious resistance; resistance to avermectin 1.2 times, 4.51 times and 1.2 times, respectively. There are 11 kinds of isozymes in the population of Estα and Estβ in Beijing population, including 4 isozymes and 7 β isozymes. There are 3 isoenzymes and 5 β isozymes in Hebei population There are three kinds of α isozymes and three kinds of β isozymes in Yunnan population. Polymorphism of esterase gene in three populations may be the result of passive transport of Plutella xylostella carrying different resistant esterase genes. Insecticide pairs may not have interactive resistance to insecticide which can cause esterase activity increase.