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稻瘟病是由真菌Magnaporthe oryzae引起的,水稻与稻瘟病菌的互作符合经典的“基因对基因”学说,抗稻瘟病基因Pi-ta可有效防治携带AVR-Pita1稻瘟菌的侵染。本研究利用位于Pi-ta基因前端的显性分子标记YL153/YL153和位于中间区域的显性分子标记YL155/YL87对39份来自美国的水稻种质进行Pi-ta基因检测,同时利用携带AVR-Pita1的稻瘟病生理小种IC-17、IB-49、IB-1、IE-1、IG-1和IH-1接种鉴定39份水稻种质的抗病性。结果表明,39份育种亲本中,分子标记YL153/YL154和YL155/YL87鉴定均持有Pi-ta基因的有26份,其中接种鉴定表现抗病的亲本有21份,感病的亲本有5份;两对分子标记鉴定不持有Pi-ta基因的有11份,其中接种鉴定表现抗病的亲本有7份,感病的亲本有4份;两对分子标记鉴定结果不一致的亲本有2份,即一对分子标记鉴定持有Pi-ta基因,另一对分子标记鉴定无Pi-ta基因,接种鉴定均表现感病,这一发现对进一步研究Pi-ta基因的功能具有重要意义。由此说明,在水稻育种中,选择抗稻瘟病亲本时,分子标记检测和接种鉴定都是重要的。
The rice blast is caused by the fungus Magnaporthe oryzae. The interaction between rice and Magnaporthe oryzae conforms to the classical “gene-to-gene” theory. The blast-resistant gene Pi-ta can effectively prevent and treat the infection with the blast fungus AVR-Pita1 . In this study, Pi-ta gene was detected in 39 rice germplasms from the United States using the dominant molecular marker YL153 / YL153 located in the front of Pi-ta gene and the dominant molecular marker YL155 / YL87 located in the middle region. At the same time, The resistance of 39 accessions to rice germplasm was inoculated with the blast physiological races IC-17, IB-49, IB-1, IE-1, IG-1 and IH- The results showed that among the 39 breeding parents, 26 were identified as Pi-ta genes by molecular markers YL153 / YL154 and YL155 / YL87, of which 21 were identified for disease resistance and 5 for susceptible parents ; 11 pairs of Pi-ta genes were identified by two pairs of molecular markers, of which 7 were resistant to disease and 4 were susceptible to inoculation; 2 were incompatible with two pairs of molecular markers , That is, a pair of molecular markers to identify the Pi-ta gene identification, another pair of molecular markers to identify non-Pi-ta gene inoculation were identified susceptible, the discovery of further study of the function of Pi-ta gene is of great significance. Thus, molecular marker detection and inoculation identification are both important in the selection of parents for blast resistance in rice breeding.