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目的:建立一个合适的乳腺癌动物模型将在研究人类乳腺癌的发生、发展、转移等方面中发挥着越来越重要的作用。7,12-二甲基苯并蒽(7,12-dimethylbenz anthracene,DMBA)在实验中能诱导大鼠产生乳腺肿瘤。树鼩的基因的结构与人类的相似程度比啮齿类动物要高,而且树鼩的自发性乳腺癌已经有被报道,因而树鼩很有可能是研究乳腺肿瘤更合适的动物模型。因此我们想用致癌剂DMBA诱导树鼩产生乳腺肿瘤而建立树鼩的乳腺肿瘤模型。方法:在这个研究中,我们采用了十只在分娩之后失去幼崽的雌树鼩,其中一半的树鼩在腰部双侧乳房的脂肪垫注射100 mg/kg的DMBA,其余的树鼩作为对照组没有作DMBA处理。对生成的肿瘤组织进行病理切片HE染色的形态特点分析以及免疫组化化学法测定Ki-67、雌激素受体、孕酮受体、人表皮生长因子受体-2、E-钙粘蛋白、P120连环蛋白的表达。结果:通过诊断在DMBA处理的树鼩中,5分之1发展浸润性导管癌,其余发展成原位导管癌。结果还证明了诱导出来的乳腺肿瘤的形态学和病理学特征与人类的浸润性导管癌相似。结论:结果显示我们采用DMBA注射失去幼崽的雌树鼩的乳腺来诱导乳腺肿瘤是有效的,诱导出来的肿瘤组织学特征与人的乳腺癌相似,诱导的肿瘤组织表达目前人常用的乳腺癌相关分子生物学标记,并且表达情况与人的乳腺癌相似。这表明了DMBA诱导树鼩乳腺癌可以提供一个适合于研究人类乳腺癌发生、发展、转移和治疗的动物模型。
Objective: To establish a suitable animal model of breast cancer will play an increasingly important role in the study of the occurrence, development and metastasis of human breast cancer. 7,12-dimethylbenzanthracene (7,12-dimethylbenz anthracene, DMBA) was able to induce rat mammary tumors in experiments. Tree shrews have a more human-like structure than rodents and the tree shrews have been reported to have spontaneous breast cancers, so shrubs are likely to be the more appropriate animal models for studying breast tumors. Therefore, we want to use the carcinogen DMBA-induced tree shrews to produce breast tumors to establish a tree shrew model of breast tumors. METHODS: In this study, we used ten female tree trunks that lost their pups after delivery. Half of the tree trunks were injected with 100 mg / kg DMBA on the fat pads of the bilateral lumbar breasts and the rest of the tree trunks were used as controls Group did not make DMBA treatment. The morphological characteristics of the tumor tissue were analyzed by HE staining and immunohistochemical methods were used to detect Ki-67, estrogen receptor, progesterone receptor, human epidermal growth factor receptor-2, E-cadherin, P120 catenin expression. RESULTS: One-fifth of DMBA-treated tree shrews developed invasive ductal carcinoma and the remainder progressed to ductal carcinoma in situ. The results also demonstrate that the morphological and pathological features of the induced breast tumors are similar to those of human invasive ductal carcinoma. CONCLUSIONS: The results showed that we used DMBA injection to induce mammary gland mammary glands that lost the female virginity of the young cubs to induce breast tumors. The induced histological features were similar to those of human breast cancer. The induced tumor tissues expressed commonly used breast cancer Related molecular biology markers and expression similar to human breast cancer. This indicates that DMBA-induced tree shrew breast cancer can provide an animal model suitable for studying the occurrence, development, metastasis and treatment of human breast cancer.