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核基质(nuclear matrix,NM)蛋白作为胞核的骨架,影响其形态特征、完整性和功能。它还与细胞分化及向恶性转化有关。此研究欲证实正常上呼吸道消化道上皮向头颈部鳞状细胞癌(squamous cell carcinoma of the head and neck,SCCHN)转化与NM蛋白成分的特异性改变有关的假说。 选择液氮保存的12例原发性SCCHN患者的手术标本及5例正常人口咽喉部粘膜标本(因阻塞性睡眠呼吸暂停综合征而手术)。标本常规解冻、组织匀浆、过滤、离心,得到胞核沉淀后依次去除可溶性细胞骨架成分、染色质、RNA及中间丝,得到NM蛋白。高分辨率二维凝胶电泳分离之(根据等电点及分子量),银染后照相作负片观察蛋白质模式。为取得标准化,每个标本
The nuclear matrix (NM) protein acts as the backbone of the nucleus, affecting its morphological characteristics, integrity and function. It is also related to cell differentiation and to malignant transformation. This study sought to confirm the hypothesis that normal upper respiratory tract epithelium is involved in the conversion of squamous cell carcinoma of the head and neck (SCCHN) to specific changes in NM protein components. Twelve surgical specimens of primary SCCHN patients preserved with liquid nitrogen and five cases of normal population throat mucosa specimens (operations due to obstructive sleep apnea syndrome) were selected. Specimens were routinely thawed, homogenized, filtered, and centrifuged to obtain nucleus precipitates. Dissolved cytoskeletal components, chromatin, RNA, and intermediate filaments were sequentially removed to obtain NM proteins. High-resolution two-dimensional gel electrophoresis separation (according to isoelectric point and molecular weight), silver stained photographs were used as negative films to observe protein patterns. For standardization, each specimen