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利用发育毒性体外评价模型开展相关安全性评价,可避免人体或动物实验耗时、费力等缺点,适用于大规模体外高通量筛选。目前常用的体外评价模型中,胚胎干细胞实验、大鼠全胚胎培养以及体外胚胎肢芽培养实验因与体内实验结果一致性较强,已得到广泛的认可和推荐。但与一般毒理学体外试验发展相比,由于发育毒性受试物在体内消化代谢路线路径的差异,使得发育毒性试验的筛选实验的发展和完善受到阻碍,因此合理的利用新技术、新方法,提高筛选体系的合理性、系统性成为未来体外评价模型的方向。
The use of developmental toxicity in vitro evaluation model to carry out relevant safety evaluation, can avoid human or animal experiments time-consuming and laborious shortcomings, suitable for large-scale high-throughput screening in vitro. Currently used in vitro evaluation model, embryonic stem cell experiments, rat whole embryo culture and in vitro embryo bud training experiments and in vivo results of strong consistency, has been widely recognized and recommended. However, compared with the development of in vitro toxicology tests in general, the development and improvement of screening experiments for developmental toxicity tests are hindered due to differences in the digestion and metabolism pathways of developmental toxicity test substances in vivo. Therefore, rational utilization of new technologies, new methods, Improve the rationality of the screening system, and systematically become the direction of the future in vitro evaluation model.