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应用花药离体培养技术 ,对水稻不同遗传背景材料的去分化和再分化能力以及花粉植株后代性状遗传变异动态进行研究 ,并分析了花粉植株后代的有关同工酶。结果表明 :1.不同遗传背景材料的愈伤组织诱导率、绿苗分化率有明显差异 ,其中 94 P 70的培养力最高 ,其次是汕优桂 99,其余未分化出绿苗。2 .花粉植株一代 ( H1)性状表现多样性 ;H2 代有少数株系的性状优于 H1代 ,发生分离 ,大多数株系的主要性状相对稳定 ,并出现优于 H1代及亲本的性状。3.花粉植株 H2 代的大多数株系的酯酶和过氧化物酶谱带数与亲本相同 ,只有个别株系与亲本不同。
In vitro anther culture techniques were used to study the dedifferentiation and redifferentiation ability of different genetic backgrounds in rice and the genetic variation of the progenies of pollen plants. The related isozymes of pollen plants were analyzed. The results showed that: 1. The callus induction rate and the differentiation rate of green plantlets were significantly different among different genetic background materials, among which, 94 P 70 had the highest culture ability, followed by Shanyougui 99 and the other non-differentiated green plants. In the H2 generation, the traits of a few strains were superior to those of the H1 generation, and the segregation occurred. The main traits of most of the strains were relatively stable and appeared better than the H1 generation and the parents. 3. Pollen plants H2 generation most of the strains of esterase and peroxidase bands with the same number of parents, only some of the strains and parents are different.