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目的观察大黄素对人胃癌MNK-45和MGC8-03细胞增殖的影响。方法采用MTT法测定MNK-45细胞和MGC8-03细胞增殖抑制率;采用AO/EB双染观察细胞形态学变化;采用流式细胞仪测定细胞周期。结果大黄素(5～80μg/mL)对MNK-45和MGC8-03细胞增殖均具有显著抑制作用(P<0.01或0.05),且药物剂量越大,作用时间越长,其抑制作用越强(P<0.01);大黄素作用于两种细胞后荧光显微镜下观察均显示细胞凋亡特征;大黄素使MNK-45和MGC8-03细胞增殖被阻滞于G0/G1期。结论大黄素能抑制人胃癌MNK-45和MGC8-03细胞增殖,诱导其凋亡,影响其细胞周期时相的分布。
Objective To observe the effect of emodin on the proliferation of human gastric cancer MNK-45 and MGC8-03 cells. Methods The proliferation inhibition rates of MNK-45 cells and MGC8-03 cells were determined by MTT assay. The morphological changes of cells were observed by AO / EB double staining. The cell cycle was determined by flow cytometry. Results Emodin (5 ~ 80μg / mL) had a significant inhibitory effect on the proliferation of MNK-45 and MGC8-03 cells (P <0.01 or 0.05). The larger the dose of drug was, the longer the effect was. P <0.01). Emodin induced apoptosis in MNK-45 and MGC8-03 cells in both G0 / G1 phase under fluorescence microscope. Conclusion Emodin can inhibit the proliferation of human gastric cancer cell line MNK-45 and MGC8-03, induce apoptosis and influence the distribution of cell cycle phases.