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目的:探讨顺铂诱导大鼠化疗损伤性卵巢功能早衰大鼠模型的可行性。方法:成熟雌性SD大鼠腹腔注射低、高剂量顺铂4.5 mg/kg(A组)、6.0 mg/kg(B组)和生理盐水(C组),每周1次,共2次,建立大鼠化疗损伤性卵巢早衰模型。检测血清FSH水平及光学显微镜下计数卵巢最大切面原始卵泡、初级卵泡、闭锁卵泡,阴道涂片观察动情周期变化。结果:A、B组大鼠动情周期均明显长于C组(P<0.05),并呈现剂量相关性改变。B组动情周期天数明显长于注射前(P<0.01);血清FSH水平A组与C组相比无统计学意义(P>0.05),B组明显高于A组和C组(P<0.05)。各顺铂组血清FSH水平注射后均较注射前明显升高(P<0.05)。腹腔注射顺铂后,A组、B组大鼠卵巢最大切面原始卵泡数和初级卵泡数均明显降低(P>0.05),而闭锁卵泡数均明显增加(P<0.05),并呈现剂量相关性改变。结论:顺铂可诱导化疗损伤性卵巢早衰。此化疗损伤性卵巢早衰大鼠模型血FSH明显升高,卵巢组织学衰退性改变与人类化疗损伤性卵巢早衰病变过程相似。
Objective: To investigate the feasibility of cisplatin-induced rat model of premature ovarian failure induced by chemotherapy. Methods: Mice were injected intraperitoneally with low and high dose cisplatin 4.5 mg / kg (group A), 6.0 mg / kg (group B) and saline (group C) once a week for 2 weeks to establish Rat model of chemotherapy induced premature ovarian failure. Serum FSH levels were measured and the ovarian primordial follicles, primary follicles, atresia follicles and vaginal smears under the light microscope were counted to observe the changes of estrous cycle. Results: The estrous cycle of rats in groups A and B were significantly longer than those in group C (P <0.05), and showed dose-related changes. The days of estrous cycle in group B were significantly longer than that in group B before injection (P <0.01). There was no significant difference in serum FSH level between group A and group C (P> 0.05) . The serum levels of FSH in cisplatin group were significantly higher than those before injection (P <0.05). After intraperitoneal injection of cisplatin, the number of primary follicles and the number of primary follicles in the largest section of ovary in group A and group B were significantly decreased (P> 0.05), while the number of atresia follicles was significantly increased (P <0.05) change. Conclusion: Cisplatin can induce chemotherapy-induced premature ovarian failure. The chemotherapy-induced premature ovarian failure in rats with premature ovarian failure FSH was significantly increased, ovarian histopathological changes and human chemotherapy in patients with premature ovarian failure lesions similar.