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目的 认识白介素 1β转化酶 (ICE)在脑缺血再灌注损伤中表达及在细胞凋亡中的作用。 方法通过阻塞大鼠双侧颈总动脉和椎动脉建立全脑缺血模型 ,使用免疫细胞化学、核酸分子原位杂交技术和原位末端标记 ,观察了ICE蛋白和基因表达变化、脑缺血后细胞凋亡的发生以及ICE基因表达与细胞凋亡的关系。结果 ICE基因在神经元内及小胶质细胞均有表达 ,分布在大脑皮层、小脑蒲肯野细胞、海马及皮层下白质。在缺血再灌注 12h后ICE基因表达增加 ,4 8～ 72h为表达高峰 ,7d表达下降。细胞凋亡在缺血再灌注12h出现 ,高峰时间也在 4 8～ 72h ,且ICE的表达在分布上与神经细胞凋亡的发生有显著相关性。结论 结果提示ICE在脑缺血再灌注中表达增加 ,可能参与神经细胞凋亡的调节。
Objective To understand the expression of interleukin-1 beta converting enzyme (ICE) in cerebral ischemia-reperfusion injury and its role in apoptosis. Methods The model of global cerebral ischemia was established by occluding bilateral common carotid arteries and vertebral arteries in rats. The changes of ICE protein and gene expression were observed by immunocytochemistry, in situ hybridization and in situ terminal labeling. After cerebral ischemia The occurrence of apoptosis and the relationship between ICE gene expression and apoptosis. Results ICE gene was expressed in neurons and microglia, distributed in cerebral cortex, cerebellum Purkinje cells, hippocampus and subcortical white matter. ICE gene expression was increased 12h after ischemia-reperfusion, peaked at 48h-72h, and decreased at 7d. Apoptosis occurred at 12h after ischemia-reperfusion, peak time was 48 ~ 72h, and the expression of ICE was significantly correlated with the occurrence of neuronal apoptosis. Conclusion The results suggest that ICE expression increases in cerebral ischemia-reperfusion, which may be involved in the regulation of neuronal apoptosis.