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目的：探讨高温诱导低分化鼻咽癌细胞系ＣＮＥ－２Ｚ凋亡的作用。方法：用激光共聚焦显微技术、核酸电泳、免疫组化等方法分析了４２．５℃高温处理６、１２、２４ｈ后鼻咽癌细胞的变化。结果：激光共焦显微镜观察可见高温处理６ｈ肿瘤细胞出现空泡，有不同程度的固缩；１２ｈ染色质浓缩缘边化，呈“半月形”、细胞出芽，出现凋亡小体，２４ｈ后大部分细胞死亡。ＤＮＡ琼脂糖凝胶电泳可见高温处理６ｈ和１２ｈ后有断裂成小分子片段，呈典型梯状图谱；２４ｈ后为不规则的图谱。ｂｃｌ－２免疫组化显示高温处理后的鼻咽癌细胞ｂｃｌ－２蛋白表达逐渐减弱。结论：高温可诱导鼻咽癌细胞的凋亡，特别是４２．５℃处理１２ｈ后明显。ｂｃｌ－２蛋白与细胞凋亡之间关系密切。
Objective: To investigate the effect of high temperature on the apoptosis of poorly differentiated nasopharyngeal carcinoma cell line CNE-2Z. Methods: The changes of nasopharyngeal carcinoma cells after heat treatment at 42.5 ℃ for 6, 12 and 24 hours were analyzed by laser confocal microscopy, nucleic acid electrophoresis and immunohistochemistry. Results: Laser confocal microscopy showed that after 6 hours of high temperature treatment, the tumor cells showed vacuolization with different degrees of condensation. At 12th hour, the chromatin margin was marginalized and showed “half-moon shape” with sprouting and apoptotic bodies. After 24 hours, Some cells died. DNA agarose gel electrophoresis showed high temperature treatment 6h and 12h after breaking into small molecule fragments, showed a typical ladder map; irregular pattern after 24h. bcl-2 immunohistochemistry showed that bcl-2 protein expression in nasopharyngeal carcinoma cells gradually decreased after heat treatment. Conclusion: High temperature can induce the apoptosis of nasopharyngeal carcinoma cells, especially at 42.5 ℃ for 12h. Bcl-2 protein and apoptosis are closely related.