探讨了高温灭菌工艺(121℃,110.35kPa)对纳米银颗粒在两种模拟体液(PBS溶液和DMEM培养液)中的分散状况的影响,并用TEM观测纳米银颗粒在两种溶液中的尺寸和分散程度及用XPS检测溶液中的纳米银颗粒的表面元素构成。结果表明,经高温灭菌处理后,纳米银颗粒在PBS溶液中的团聚程度明显增加;但由于纳米银颗粒表面在DMEM培养液中会发生蛋白质和氨基酸吸附,形成覆蛋白膜的纳米银颗粒,因此经高温灭菌处理后,纳米银颗粒依然能够在DMEM培养液中以单颗粒形式均匀存在。这一结果说明在DMEM培养液中的纳米银颗粒即使经过高温灭菌工艺处理,依然可以保持很好的分散状态,因此DMEM是适合于纳米银颗粒下一步临床应用的分散剂。 The effects of high temperature sterilization process (121 ℃, 110.35kPa) on the dispersion of nano-silver particles in two simulated body fluids (PBS and DMEM) were investigated. The size of the nano-silver particles in the two solutions And the degree of dispersion and XPS detection of the solution of the nano-silver particles surface elements. The results showed that after the high temperature sterilization, the agglomeration degree of nano silver particles in PBS solution increased obviously. However, due to the protein and amino acid adsorption on the surface of nano silver particles in DMEM culture medium, Therefore, after high-temperature sterilization, the nano-silver particles can still exist in DMEM culture medium as single particles. This result shows that the DMEM is still suitable for the further clinical application of nano-silver particles in the DMEM culture medium even after being treated by high-temperature sterilization process.