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目的:对甘草芫花合煎液和合并液进行HPLC测定,分析两种提取液色谱图差异,探讨甘草芫花配伍产生毒性的可能机理。方法:制备甘草芫花提取液、甘草芫花合煎液及1∶1合并液,分别进行HPLC测定。结果:甘草芫花合煎液与合并液色谱图存在一定差异,合并液色谱图与芫花色谱图在12 min均有一个吸收峰,而合煎液的色谱图没有发现类似峰,但在10.5 min出现一个新的吸收峰,此吸收峰在甘草与芫花的色谱图中均未发现。结论:甘草芫花合并煎煮与分别煎煮后成分具有差异,这种差异同甘草与芫花的配伍合理性之间存在的关系,以及因煎煮工艺不同而造成毒性程度的不同等问题,值得进一步研究。
OBJECTIVE: To determine the licorice decoction decoction and the combined solution by HPLC, and to analyze the chromatogram differences between the two extracts, and to explore the possible mechanism of the toxicity of licorice decoction. Methods: The extracts of Glycyrrhiza uralensis Fisch, Glycyrrhiza uralensis Fisch combined liquid and 1:1 mixture were prepared and determined by HPLC. RESULTS: There were some differences in the chromatograms of the decoction of licorice extract and the combined solution. The chromatograms of the combined liquid chromatograms and the chromatograms of the corkscrew had an absorption peak at 12 min. However, no similar peak was found in the chromatogram of the combined decoction, but at 10.5. A new absorption peak appeared in min. This absorption peak was not found in the chromatograms of licorice and alfalfa. Conclusion: There is a difference in the composition of licorice decoction combined with decoction and decocting respectively. This difference is related to the relationship between the rationality of the compatibility of licorice and alfalfa, and the difference in the degree of toxicity caused by different decoction processes. It is worth further study.