论文部分内容阅读
目的 观察血管紧张素 ( 1 7) [angiotensin ( 1 7) ,Ang ( 1 7) ]对二肾一夹 (two kidney ,onecliphypertension ,2K1C)高血压大鼠肾脏结构和功能的影响。方法 采用微渗泵植入技术 ,复制Ang ( 1 7)对高血压大鼠干预模型 ,光镜下观察肾脏病理变化 ;考马斯亮蓝法测定尿蛋白 ,全自动生化分析仪测定血肌酐、尿素氮。结果 Ang ( 1 7)干预 2 8d后 ,2K1C高血压大鼠肾纤维化明显减轻 (光镜 ) ,尿蛋白 (g L)显著减少 ( 0 2 63± 0 172vs 0 5 79± 0 15 8,P <0 0 1) ,对血肌酐( 5 2 9± 19 8vs 5 3 5 79± 2 4 3 ,P >0 0 5 )、尿素氮 ( 4 4± 2 6vs 4 2± 1 8,P >0 0 5 )无显著影响。结论 Ang ( 1 7)对 2K1C高血压大鼠肾纤维化具有一定保护作用 ,并减轻蛋白尿
Objective To investigate the effects of angiotensin (17) and Ang (17) on the structure and function of kidneys in two kidney (IKH) hypertensive rats. Methods Microinvasive implantation technique was used to duplicate Ang (17) model of hypertension in rats. The pathological changes of kidney were observed under light microscope. Proteinuria was measured by Coomassie Brilliant Blue method. Serum creatinine and urea nitrogen were measured by automatic biochemical analyzer. . Results Angiotensin II (Ang II) treatment significantly reduced renal fibrosis (P <0.05) and urinary protein (g L) in 2K1C hypertensive rats at 28 days (0 2 63 ± 0 172 vs 0 5 79 ± 0 15 8, P (P0.05), serum creatinine (529 ± 19 8vs5 3 5 79 ± 2 43, P 0 05), urea nitrogen (4 4 ± 2 6vs 4 2 ± 1 8, P 0 0 1) 5) no significant effect. Conclusion Ang (17) can protect renal fibrosis in 2K1C hypertensive rats and relieve proteinuria