目的 :探讨建立人鼻粘膜呼吸区上皮细胞原代培养模型和纤毛运动频率测量的方法。方法 :利用酶消化法分离、以无血清生长因子培养基培养人鼻粘膜上皮细胞。用电视显微镜法观察并记录纤毛运动。结果 :培养的鼻粘膜呼吸上皮细胞在接种后 2 4 h贴壁 ,6～ 8d汇合 ,成活 1 6d,因实验需要结束培养。纤毛细胞纤毛摆动活跃 ,杯状细胞既分泌酸性粘多糖也分泌中性粘多糖 ,所培养细胞 2 3对 46条染色体正常。用电视显微镜法测得 2 9例1 45个人鼻粘膜细胞的纤毛运动频率均值为 ( 4 1 1± 2 4 )次 /min。结论 :酶消化法分离、无血清生长因子培养基培养 ,可以建立人鼻粘膜呼吸区上皮细胞的原代培养模型 ;电视显微镜法可以用于纤毛运动频率测量 Objective: To explore the method of establishing a primary culture model of human nasal mucosa respiratory epithelial cells and measuring the frequency of ciliary movement. Methods: Human nasal mucosal epithelial cells were cultured in serum - free growth medium with enzyme digestion. Ciliary movement was observed and recorded using a television microscope. Results: The cultured nasal mucosa respiration epithelial cells adherent at 24 hrs after inoculation, confluent 6 ~ 8 d, and survived for 16 days. Ciliated cells cilia active swing, goblet cells secrete both acidic mucopolysaccharide and neutral mucopolysaccharide, the cultured cells 23 to 46 chromosomes normal. The average ciliary movement frequency of 29 patients with 45 human nasal mucosa measured by TV microscope was (4 1 1 ± 2 4) times / min. Conclusion: The culture of human nasal mucosa respiratory zone epithelial cells can be established by enzymatic digestion and culture with serum-free growth factor. The microscope can be used to measure the ciliary movement frequency