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目的检测皮肤切创愈合过程中α7nAChR的表达及其时间规律性变化。方法建立小鼠皮肤切创模型,应用免疫组织化学技术及Western blot方法检测切创后不同时间段皮肤中α7nAChR的表达。结果免疫组织化学结果显示,对照组α7nAChR表达于表皮、毛囊、皮脂腺、血管内皮及真皮中少数的成纤维细胞;伤后6～12h切创皮肤损伤区及周边区可见少量多核粒细胞和单核细胞表达α7nAChR,1～3d以单核细胞阳性表达为主,5～14d以成纤维细胞为主。α7nAChR阳性细胞率于伤后1d开始升高,伤后7d达最高峰,随后逐渐下降。经Western blot检测显示,对照组及各实验组均有α7nAChR阳性条带,其中伤后7d为α7nAChR表达高峰。结论α7nAChR在小鼠皮肤切创愈合过程中呈现一定的时序性变化。
Objective To detect the expression of α7 nAChR and its time-dependent changes during incised wound healing. Methods The cutaneous incision model in mice was established. The expression of α7 nAChR in skin at different time after incision was detected by immunohistochemistry and Western blot. Results The results of immunohistochemistry showed that α7nAChR in the control group was expressed in a few fibroblasts in the epidermis, hair follicles, sebaceous glands, vascular endothelium and dermis. A small amount of multinucleated granulocytes and mononuclear cells Cells expressed α7nAChR, 1 ~ 3d monocyte positive expression mainly 5 ~ 14d to fibroblasts. The positive rate of α7nAChR positive cells increased from the 1st day after injury and reached its peak on the 7th day after injury, then decreased gradually. Western blot showed that the control group and each experimental group have α7nAChR positive bands, in which 7d after injury the expression of α7nAChR peak. Conclusions α7nAChR shows some time-dependent changes during incised wound healing in mice.