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为观察乙肝病毒 Ｈ Ｂｓ Ａｇ 基因疫苗免疫小鼠后的细胞及体液免疫应答，将该疫苗定向克隆于质粒ｐｃ Ｄ Ｎ Ａ３ 巨细胞病毒启动子下游，构建能在真核细胞内表达的重组基因疫苗ｐｃ Ｄ Ｎ Ａ Ｈ Ｂｓ Ａｇ 。将此疫苗通过多点肌肉注射免疫 Ｂａｌｂ／ Ｃ 小鼠后第六周，实验组小鼠均出现抗－ Ｈ Ｂｓ Ｉｇ Ｇ 阳性，其抗体水平明显高于对照组，同时 Ｔｈ１ 免疫应答相关的细胞因子 Ｉ Ｌ２ 及γ干扰素水平也明显高于对照组，表明本文构建的基因疫苗ｐｃ Ｄ Ｎ Ａ３ Ｈ Ｂｓ Ａｇ 能诱导 Ｂａｌｂ／ Ｃ 小鼠产生良好的细胞及体液免疫应答。
To observe the cellular and humoral immune response of mice immunized with HBsAg gene vaccine of hepatitis B virus, the vaccine was cloned into the downstream of the promoter of plasmid pc D N A3 cytomegalovirus to construct a recombinant gene vaccine capable of expression in eukaryotic cells pc D N A H Bs Ag. At the sixth week after immunization of Balb / C mice with this vaccine through multi-point intramuscular injection, all the mice in the experimental group showed positive anti-H Bs Ig G and their antibody levels were significantly higher than those in the control group. Th1 immune response-related cytokines I L 2 and γ interferon levels were significantly higher than the control group, indicating that the gene vaccine pcD N A3 H Bs Ag constructed in this paper can induce Balb / C mice to produce good cellular and humoral immune response.