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目的研究家蝇抗药性产生机制,为选择快速准确的监测技术和科学用药提供理论依据。方法通过对实验室培育的抗高效氯氰菊酯品系、抗性衰减品系及敏感品系家蝇的谷胱甘肽-过氧化物酶(GSH-PX)的活性及SDS-聚丙烯酰胺凝胶电泳分析,观察过氧化物酶对家蝇抗性产生的作用。结果抗性家蝇GSH-PX的活性明显增高,抗性品系家蝇相对活性达到165.89%,抗性衰减品系活性降低到70.13%。3个家蝇品系的电泳图谱中6组条带有较明显的差别。结论抗高效氯氰菊酯家蝇的过氧化物酶性质发生改变,对家蝇的抗药性产生可能起比较重要的作用。
Objective To study the mechanism of Musca domestica drug resistance and provide a theoretical basis for selecting rapid and accurate monitoring techniques and scientific medication. Methods The activities of glutathione peroxidase (GSH-PX) and SDS-polyacrylamide gel electrophoresis (SDS-polyacrylamide gel electrophoresis) were determined in laboratory-developed cypermethrin resistant strains and susceptible strains of housefly The effect of peroxidase on housefly resistance. As a result, the activity of GSH-PX in resistant housefly was significantly increased, the relative activity of housefly in the resistant strain reached 165.89%, and the activity of the resistant attenuated strain decreased to 70.13%. There were obvious differences among the 6 bands in the electrophoresis patterns of 3 housefly strains. Conclusion The peroxidase property of housefly resistant to beta-cypermethrin is changed, which may play an important role in the drug resistance of housefly.