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目的探讨肝细胞肝癌（ＨＣＣ）中端粒酶逆转录酶（ＨＴＥＲＴ）ｍＲＮＡ表达意义及其与肿瘤抑制基因ｐ５３的相关性。方法收集３４例ＨＣＣ标本，应用ＲＮＡ－ＲＮＡ原位杂交技术检测ＨＴＥＲＴ ｍＲＮＡ表达，观察其与病理特征间的关系；用免疫组织化学技术检测ｐ５３蛋白，比较ＨＴＥＲＴ与ｐ５３表达间的关系。结果３０／３４例（８８．２％） ＨＣＣ中癌细胞ＨＴＥＲＴ ｍＲＮＡ呈阳性表达，其中小肝癌６例均为阳性；分化差、肝内外转移、包膜不完整、癌旁有活动性病变及ＨＢｓＡｇ阳性者ＨＴＥＲＴ ｍＲＮＡ表达较高；侵袭灶前沿癌细胞及少数非典型性增生的肝细胞中常见阳性。 ＨＴＥＲＴ ｍＲＮＡ和ｐ５３表达一致（均阳或均阴）者占７９．４％，两者表达具有相关性， ｐ５３阳性者 ＨＴＥＲＴ ｍＲＮＡ表达较强（Ｐ＜０．０５）。正常肝组织 ＨＴＥＲＴ ｍＲＮＡ呈阴性。结论 ＨＣＣ中 ＨＴＥＲＴ ｍＲＮＡ高表达； ＨＴＥＲＴ ｍＲＮＡ表达与ＨＣＣ发生、演进有关； ｐ５３可能参与ＨＴＥＲＴ ｍＲＮＡ的表达调控。
Objective To investigate the expression of telomerase reverse transcriptase (HTERT) mRNA in hepatocellular carcinoma (HCC) and its correlation with tumor suppressor gene p53. Methods Thirty-four cases of HCC specimens were collected and the expression of HTERT mRNA was detected by RNA-RNA in situ hybridization. The relationship between the expression of HTERT mRNA and pathological characteristics was examined. The p53 protein was detected by immunohistochemistry and the relationship between HTERT and p53 expression was compared. Results The expression of HTERT mRNA in HCC cells was positive in 30/34 cases (88.2%). Among them, 6 cases of small HCC were positive; poorly differentiated, intrahepatic/extracellular metastasis, incomplete capsule, active lesion near the tumor and HBsAg The positive expression of HTERT mRNA was higher in positive patients, and positive in invasive cancerous lesions and a few atypical hyperplastic hepatocytes. HTERT mRNA and p53 expression were consistent (mean positive or negative) accounted for 79.4%, the expression of the two has a correlation, p53 positive HTERT mRNA expression was strong (P <0.05). Normal liver tissue is negative for HTERT mRNA. Conclusion The expression of HTERT mRNA is highly expressed in HCC. The expression of HTERT mRNA is related to the occurrence and progression of HCC. p53 may be involved in the regulation of HTERT mRNA expression.