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应用清醒大鼠脑微透析技术以选择性可溶性鸟苷酸环化酶抑制剂 1 H- [1 ,2 ,4]口恶二唑 [4,3- a]喹喔啉 - 1 -酮 ( ODQ)为工具药研究大鼠小脑 NO-c GMP神经通路 .小脑内局部灌流 ODQ( 5,1 0 ,50 ,1 0 0 μmol· L-1,5μL·min-1)可剂量依赖性降低细胞外基础 c GMP水平 ,最大可使细胞外基础 c GMP水平降低 80 % .ODQ1 0 0 μmol· L-1可完全对抗N -甲基 - D-天冬氨酸 ( NMDA,2 0 0μmol· L-1)或α-氨基羟甲基异口恶唑丙酸 ( AMPA,1 0 0μmol· L-1)引起的细胞外 c GMP水平增加 ,ODQ也可抑制 NO供体 S-亚硝基 - N-乙酰青霉胺 ( SNAP,1 .5mmol·L-1)引起的细胞外 c GMP水平增加 .说明在基础条件下小脑内 c GMP80 %来源于可溶性鸟苷酸环化酶激活 .进一步证明了 NO通过激活可溶性鸟苷酸环化酶使 c GMP增加 .
Using conscious rat brain microdialysis technique, a selective soluble guanylate cyclase inhibitor 1 H- [1,2,4] oxadiazole [4,3- a] quinoxalin-1-one (ODQ ) Was used as a tool to study the cerebellar NO-c GMP neural pathway in rat cerebellum.The ODQ (5,1 0,50,50 and 100 μmol · L -1,5 μL · min -1) in the cerebellum decreased dose-dependently The basal c GMP level decreased the extracellular basal c GMP level by 80% .ODQ1 0 μmol · L-1 could completely antagonize the N-methyl-D-aspartate (NMDA, 200μmol · L-1 ) Or extracellular c GMP levels induced by α-amino hydroxymethylisoxazole-propionic acid (AMPA, 100 μmol · L -1) increased ODQ also inhibited NO donor S-nitroso-N-acetyl Penicillium (SNAP, 1.5mmol · L-1) caused by increased extracellular c GMP levels, indicating that under basic conditions cGMP 80% of cerebellum derived from soluble guanylate cyclase activation further evidence that NO by activation Soluble guanylate cyclase increases cGMP.