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目的 探讨阿伐他汀对幼年大鼠心肌成纤维细胞 (CF)增殖和胶原合成的影响。方法 用消化法培养新生SD大鼠的CF ,采用3H 胸腺嘧啶核苷 ( 3H TdR)掺入法测定CF的DNA合成功能 ,四氮唑蓝 (MTT)比色法测定细胞增殖 ,流式细胞分析仪技术检测细胞周期 ,3H 脯氨酸掺入法测定胶原合成 ,分别观察不同浓度阿伐他汀对CF增殖和胶原合成的影响。结果 ( 1)阿伐他汀以浓度依赖的方式抑制CF的3H TdR掺入率。其中 ,10 -7、10 -6、10 -5和 10 -4 mol/L组的3H TdR掺入率分别为(cpm/ 5 0 0 0细胞 ) 314± 6 8、2 5 3± 5 2、2 0 1± 5 3和 170± 48,显著低于对照组 ( 378± 6 5 ) (F =16 912 ,P <0 0 0 1)。 ( 2 )随着阿伐他汀浓度的增高 ,CF的MTT比色法A4 90 值呈明显的递减趋势 ,其中 10 -7、10 -6、10 -5和 10 -4 mol/L组的A4 90 值分别为 0 2 88± 0 0 0 8、0 2 5 2± 0 0 0 7、0 2 2 5± 0 0 0 8和 0 2 16± 0 0 13,与对照组 ( 0 311± 0 0 0 5 )相比 ,差异有非常显著意义 (P均 <0 0 1)。 ( 3)G0 /G1期细胞百分率随阿伐他汀浓度的增高而呈递增趋势 ,S期G2 /M期细胞百分率和增殖指数呈递减趋势 ,其中 10 -7、10 -6、10 -5和10 -4 mol/L组与对照组比较 ,差异有非常显著意义 (P均 <0 0 1)。 ( 4)CF的3H 脯?
Objective To investigate the effect of atorvastatin on the proliferation and collagen synthesis of cardiac fibroblasts (CFs) in young rats. Methods The CF of neonatal SD rats was cultured by digestion, the DNA synthesis function of CF was determined by 3H TdR incorporation, the proliferation of CF was measured by MTT colorimetric assay, The cell cycle was detected by the instrument and 3H-proline incorporation method was used to determine the collagen synthesis. The effects of different concentrations of atorvastatin on CF proliferation and collagen synthesis were observed. Results (1) Atorvastatin inhibited the incorporation of 3H TdR of CF in a concentration-dependent manner. The incorporation rates of 3H TdR in 10 -7, 10 -6, 10 -5 and 10 -4 mol / L groups were 314 ± 6 8, 25 3 ± 5 2 (cpm / 500 cells) 2 0 1 ± 5 3 and 170 ± 48, which were significantly lower than those in the control group (378 ± 65) (F = 16 912, P 0 01). (2) With the increase of atorvastatin concentration, the value of MT90 colorimetric method for CF showed a decreasing trend, in which the A490 of 10 -7, 10 -6, 10 -5 and 10 -4 mol / L groups Values were 0 2 88 ± 0 0 0 8,0 2 5 2 ± 0 0 0 7,0 2 2 5 ± 0 0 0 8 and 0 2 16 ± 0 0 13, respectively, compared with the control group (0 311 ± 0 0 0 5), the difference was significant (P <0.01). (3) The percentage of cells in G0 / G1 phase increased with the increase of atorvastatin concentration, the percentage of cells in G2 / M phase and the proliferation index decreased gradually in S phase, of which 10 -7, 10 -6, 10 -5 and 10 -4 mol / L group compared with the control group, the difference was significant (P all <0.01). (4) CF3H preserved?