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许多实验研究工作需从同一个体连续采血,现介绍一种由日本学者[1]开发,并经我们在实践中加以改良的不需麻醉及固定,可在不同时间连续采血的实用方法。我们主要把原法中的填充剂PVP(聚乙烯吡咯啉酮)改由甘油代替,因为PVP易干燥凝固,配制也费时。1 材料和方法1.1 材料 (1)埋管
Many experimental studies require continuous blood sampling from the same individual. A practical method developed by a Japanese scholar [1] and improved in practice without continuous anesthesia and fixation and continuous blood sampling at different times is presented. We mainly replace the original method of filler PVP (polyvinyl pyrrolidone) replaced by glycerol, because PVP easy to dry coagulation, preparation is also time-consuming. 1 Materials and methods 1.1 Materials (1) buried pipe