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肿瘤细胞中存在的染色体区杂合性丢失(LOH)预示着新抑癌基因存在的可能。宫颈癌中存在3p14,3p21-22,3p25,6p21-23和11q22-q24等多个高度LOH区,并在部分区域中鉴定出宫颈癌相关新抑癌基因,如FHIT,RASSF1A和TSLC1等。但因单纯依赖基于微卫星标记的LOH分析和基因突变分析,忽略了染色体纯合缺失及基因启动子区高甲基化所引起的抑癌基因失活,因而鉴定的新抑癌基因甚少。目前LOH、突变分析、DNA甲基化检测、CGH等多种技术的综合应用将为宫颈癌相关新抑癌基因的鉴定带来更多希望。
Loss of heterozygosity (LOH) in the chromosomal region present in tumor cells predicts the possibility of a new tumor suppressor gene. There are many highly LOH regions of 3p14, 3p21-22, 3p25, 6p21-23 and 11q22-q24 in cervical cancer, and some new tumor suppressor genes of cervical cancer such as FHIT, RASSF1A and TSLC1 were identified in some regions. However, due to relying solely on microsatellite markers LOH analysis and gene mutation analysis, ignoring the chromosomal homozygosity loss and gene promoter hypermethylation caused by tumor suppressor gene inactivation, and thus identify a small number of new tumor suppressor genes. The current comprehensive application of LOH, mutation analysis, DNA methylation detection, CGH and other technologies will bring more hope for the identification of new tumor suppressor genes related to cervical cancer.