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目的 :研究巨噬细胞游走抑制因子 (MIF)基因修饰的 FBL 3红白血病细胞的生物学性质变化 ,为探讨其体内诱导抗肿瘤免疫反应的作用奠定基础。 方法 :以腺病毒介导 ,将 MIF基因导入小鼠 FBL 3红白血病细胞 ,观察其体外生物学性质变化。将 MIF基因修饰的 FBL 3细胞 (MIF- FBL 3)接种小鼠体内 ,观察其致瘤性和局部病理学变化 ,同时检测细胞因子 m RNA表达。 结果 :腺病毒能有效地将 MIF基因导入 FBL 3细胞 ,MIF的分泌可持续 10 d。 MIF- FBL 3细胞形态、增殖能力及表面MHC- ,MHC- ,B7- 1,B7- 2 ,ICAM- 1等分子的表达与野生型 FBL 3细胞相比无明显变化 ,但在小鼠体内的致瘤性降低 ,小鼠存活期延长 ,肿瘤局部可检测出 m TNF-α m RNA和 m L tn m RNA的表达 ,并有明显的单核和淋巴细胞浸润。 结论 :MIF-FBL 3细胞体外生物学性质无明显改变 ,但可能通过分泌 MIF在体内激活抗肿瘤免疫功能。
Objective: To study the biological properties of FBL 3 erythroleukemia cells modified by macrophage migration inhibitory factor (MIF) gene and lay a foundation for exploring the role of anti-tumor immune responses induced in vivo. METHODS: Adenovirus-mediated introduction of MIF gene into mouse FBL 3 erythroleukemia cells was performed to observe changes in biological properties in vitro. MIF gene-modified FBL 3 cells (MIF-FBL 3) were inoculated into mice to observe its tumorigenicity and local pathological changes, and to detect the expression of cytokine m RNA. Results: Adenovirus can effectively import MIF gene into FBL 3 cells, and the secretion of MIF can last 10 days. MIF-FBL 3 cell morphology, proliferation and surface expression of MHC-, MHC-, B7-1, B7-2, ICAM-1 and other molecules showed no significant changes compared to wild-type FBL3 cells, but in mice. The tumorigenicity was reduced and the survival time of mice was prolonged. The expression of mTNF-α m RNA and m L tn m RNA could be detected locally in the tumor, and obvious mononuclear and lymphocyte infiltration were observed. Conclusion : The biological properties of MIF-FBL 3 cells have no obvious changes in vitro, but it may activate anti-tumor immune function in vivo by secreting MIF.