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以抗草甘膦转EPSPS基因大豆和不同生长类型的非转基因大豆为材料,通过表型筛选及PCR分子检测,分析不同方向和距离条件下转基因大豆中外源基因通过花粉向非转基因大豆的漂移率,为制定转基因大豆安全种植隔离措施提供依据。结果表明,不同生长类型大豆的基因漂移率明显不同,以育成品系ZZH015最高,在70 612株中的漂移率为0.45%;半蔓生地方品种DPZ722次之,164 449株中漂移率为0.01%,而195 088株野生大豆YDD080中没有检测到基因漂移。方差分析表明,漂移率在不同距离和方向条件下均有显著差异,随着距离的增加不断递减。在5 m处漂移率为0.03%,而在29 m处降至0.001%。基因漂移在下风口方向发生较多,表明风媒可能是影响基因漂移分布的重要因素。如果以1%基因漂移率为阈值,无论是自然条件下还是随机选取1 000粒检测,漂移率都低于阈值,表明转EPSPS基因大豆的基因漂移可以忽略不计。
Glyphosate-resistant transgenic EPSPS soybean and different growth types of non-genetically modified soybean were used as materials. Phenotypic screening and PCR detection were used to analyze the effects of different orientations and distances on the migration rate of foreign genes from transgenic soybean to non-transgenic soybean , To provide a basis for establishing safe isolation and isolation measures of transgenic soybean. The results showed that the gene drift rate of different growing soybean was significantly different. The highest yield was ZZH015, the drift rate was 0.45% among 70 612 strains, DPZ722 followed by that of 164 449 strains, and the drift rate was 0.01% No gene drift was detected in 195 088 wild soybean YDD080. Analysis of variance showed that the drift rate under different distances and directions were significantly different, with decreasing distance increasing. The drift rate was 0.03% at 5 m and dropped to 0.001% at 29 m. The gene drift occurred more in the lower tuyere, which indicated that wind transport may be an important factor affecting the distribution of gene drift. If the 1% gene drift rate is the threshold value, the drift rate is lower than the threshold value, no matter under the natural conditions or the random selection of 1000, which indicates that the genetic drift of soybean transformed with EPSPS gene is negligible.