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生物自显影是一种在色谱上显示抗菌活性的方法,已广泛地用于寻找新的抗菌剂.几乎所有已知方法都是以琼脂扩散技术为基础,抗菌化合物通过扩散从色层板转移到已接种的琼脂板上,再通过适当活体染色,使抑制环显示出来.该程序具有某些缺点而且需要使用合适的微生物仪器.本文介绍一种在薄层上简易、直接检测抗菌化合物的生物自显影方法.检测原理:把悬浮在肉汤里的微生物加到经展开过的薄层板上,置潮湿空气中孵育.然后通过脱氢酶活性测定剂即一种四鎓盐显示抑制环带.由于活性细菌的代谢作用把四鎓盐转变成为相应的深色甲?,因此抗菌化合物对有色背景呈现亮点.
Biomolecular imaging is a method of displaying antibacterial activity on a chromatographic basis and has been used extensively to find new antibacterial agents.All practically known methods are based on agar diffusion techniques and the antimicrobial compound is transferred from the chromatography plate to The inoculated agar plates are then stained by the appropriate living organism to visualize the inhibition loop.This procedure has certain disadvantages and requires the use of suitable microbial instruments.This article presents a simple and direct bioassay detection of antibacterial compounds on a thin layer Method of detection Detection principle: The microorganisms suspended in the broth are applied to the expanded thin plate and incubated in humid air, and then the annulus is inhibited by dehydrogenase activity measuring reagent, a tetrakisonium salt. Due to the metabolic activity of the active bacteria, the onium salt is converted into the corresponding dark color A, so the antibacterial compound shows a bright spot on a colored background.