We report here a seed transformation of sonication-assisted, no-tissue culture to rapidly produce transgenic Brassica napus plants. This method comprises the steps of treating seeds by ultrasonic wave, inoculating Agrobacterium tumefaciens with a recombinant ChIFN-α gene and germinating directly of treatment seed on wet filter papers. The obtained transformants were verified by GUS histochemical assay and nested PCR amplification. It suggests that seed transformation has a potential use in genetic transformation of rape. We report here a seed transformation of sonication-assisted, no-tissue culture to rapidly produce transgenic Brassica napus plants. This method comprises steps of treating seeds by ultrasonic wave, inoculating Agrobacterium tumefaciens with a recombinant ChIFN-α gene and germinating directly of treatment Seed acquired on wet filter papers. The obtained transformants were verified by GUS histochemical assay and nested PCR amplification. It suggests that seed transformation has a potential use in genetic transformation of rape.