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(一)为了防止瓶栽灵芝的杂菌污染(从接种后到菌丝铺满之前,是污染率最高的时期),可采取以下办法: 1.抢温接种,即灭菌后的培养料温度降至25—30℃时,及时接种。 2.活化菌种,即接种前,把二级菌种放入温箱中1—2天。 (二)瓶栽灵芝的培养基,在高压灭菌后常出现表面干燥现象,致使接种后的菌丝生长缓慢,大大增加了污染的机会。解决的办法是: 1.封瓶口时,在牛皮纸外面再包一层塑料薄膜(为降低成本,可不用纱布或棉塞封口)。 2.先用无菌水湿润培养基表层后再接种。
(A) In order to prevent the fungus contamination of bottle-planting Ganoderma lucidum (from the time of inoculation to mycelium confluence, is the period of the highest pollution rate), can take the following measures: 1. grab warm vaccination, that is, Reduced to 25-30 ℃, timely inoculation. 2. Activated bacteria species, that is, before inoculation, the second species into the incubator 1-2 days. (B) bottled Ganoderma lucidum medium, often after autoclaving surface dry phenomenon, resulting in slow growth of mycelial inoculation, which greatly increased the chance of pollution. The solution is: 1. When sealing the bottle mouth, put a layer of plastic film outside the kraft paper (to reduce the cost, no gauze or tampon sealing). 2. Wet the medium surface with sterile water and inoculate it.