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目的通过比较胶原诱导(CIA)和人肿瘤坏死因子α转基因(hTNFα-Tg)小鼠关节炎模型的临床特征,为炎症性关节炎疾病的致病机理和药物筛选研究提供合适的模式动物。方法分别采用鸡Ⅱ型胶原注射DBA/1小鼠建立经典的类风湿性关节炎(RA)模型,利用人肿瘤坏死因子α注射FVB小鼠建立关节炎转基因小鼠模型,通过关节表观特征观察、关节炎指数分析和病理观察评分对炎症性关节炎模型进行评价。结果在炎症细胞浸润方面,CIA模型主要有中性粒细胞浸润,hTNFα-Tg模型主要是淋巴细胞浸润,且差异有统计学意义(P<0.05);在细胞增殖方面,两种模型都显示滑膜细胞的增生,与CIA模型相比,hTNFα-Tg模型滑膜增生最为严重,差异有统计学意义(P<0.05);在骨侵蚀方面,两种模型都显示增生滑膜对骨的侵蚀,两种模型比较,差异无统计学意义(P>0.05)。结论两种关节炎模型均在一定程度上模拟人关节炎的临床症状,但也存在着差异。CIA模型造模时间长,能较好模拟RA临床特征;hTNFα-Tg模型属于自发型,表现为滑膜不断增生,符合RA晚期临床特征。
Objective To compare the clinical features of arthritis models induced by collagen-induced (CIA) and human tumor necrosis factor-α (Tg) transgenic mice (hTNFα-Tg) and to provide a suitable model animal for studying the pathogenesis and drug screening of inflammatory arthritis. Methods The classical rheumatoid arthritis (RA) model was established by injecting type Ⅱ collagen into DBA / 1 mice. FVB mice were injected with human tumor necrosis factor α to establish arthritis transgenic mice model. , Arthritis index analysis and pathological score were used to evaluate the model of inflammatory arthritis. Results In infiltration of inflammatory cells, there were neutrophil infiltration in CIA model and lymphocytic infiltration in hTNFα-Tg model (P <0.05). In terms of cell proliferation, both of the models showed gliding Compared with CIA model, synovial hyperplasia of hTNFα-Tg model was the most serious (P <0.05). In terms of bone erosion, both models showed that the proliferation of synovial membrane on bone erosion, There was no significant difference between the two models (P> 0.05). Conclusion Both arthritis models simulate the clinical symptoms of human arthritis to some extent, but there are also differences. CIA model modeling a long time, can better simulate the clinical features of RA; hTNFα-Tg model is spontaneous, showing the proliferation of synovial membrane, consistent with the clinical features of RA late.