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目的研究红细胞密度与天龄的相关性,不同密度红细胞所对应的平均天龄。方法采用非连续密度梯度分离方法,将全血红细胞分为6个不同密度的红细胞部分。测定不同部分红细胞丙酮酸激酶活性,通过各部分丙酮酸激酶活性与全血红细胞丙酮酸激酶活性的比值(Ratio)推测各部分红细胞平均天龄;测定各部分红细胞膜表面磷脂酰丝氨酸(PS)表达阳性率,来反映红细胞密度与衰老(天龄)的相关性。结果根据红细胞比值,各部分红细胞平均天龄由低密度至高密度依次为11.5、466、3.87、4.6、80.5、102.2 d。随着红细胞密度的增加,PS表达阳性率也逐渐增高。结论不同密度的红细胞代表不同的细胞天龄,采用密度梯度分离方法可以分离出不同天龄段的红细胞,为进一步研究红细胞天龄与功能的相关性提供了方法学基础。
Objective To study the correlation between erythrocyte density and age, and the average age at different densities of erythrocytes. Methods Non-continuous density gradient method was used to divide whole blood red blood cells into 6 red blood cells with different densities. The erythrocyte pyruvate kinase activity of different parts of the erythrocytes was assayed. The ratio of pyruvate kinase activity to pyruvate kinase activity of whole blood erythrocytes was used to estimate the average age of erythrocytes. The expression of phosphatidylserine (PS) Positive rate, to reflect the red blood cell density and aging (age) of the correlation. Results According to the ratio of erythrocytes, the average age of red blood cells of each part from low to high density was 11.5,466,3.87,4.6,80.5,102.2 d. With the increase of erythrocyte density, the positive rate of PS expression also gradually increased. Conclusion Different density erythrocytes represent different ages of cells. Density gradient method can be used to separate erythrocytes of different age groups, providing a methodological basis for further study on the correlation between erythrocyte age and function.