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以NH4NO3作为变量,试验设7个处理,以普良尼柯夫配方营养液进行试验来研究氮素对芥菜幼苗生长及酶活性的影响。水培试验结果表明,芥菜幼苗移栽后14 d,营养液中的总氮量为9 mmol.L-1(N3处理)的株高、叶数、最大叶长和最大叶宽均最大,分别为4.03 cm、3.00片.株-1、2.43 cm和1.08 cm,与N0处理的差异均达到极显著水平。芥菜苗移栽后42 d,以N3处理的株高、叶数、最大叶长、地上部、地下部、总生物量、根系活跃吸收面积和最长根长均最多,分别为4.30 cm、5.00片.株-1、3.36 cm、367.87 mg、27.13 mg、395.00 mg、53.03%和6.03 cm,它们与N0的差异均达到极显著水平,除最大叶长与N0处理的差异达到显著水平外。N3处理叶片的CAT和POD活性均较高,分别为16.89 U.mg-1.min-1和134.27 U.mg-1.min-1,MDA含量最小(2.29 nmol.g-1),与N0的差异均达到极显著水平。因此,在本试验条件上,营养液中的总氮量为9 mmol.L-1(N3)处理芥菜的幼苗生长和酶活性指标最好。建议营养液中施总氮量为9 mmol.L-1。
With NH4NO3 as a variable, seven treatments were set up to study the effects of nitrogen on the growth and enzyme activities of Brassica juncea seedlings with Plennikov nutrient solution. The results of hydroponic experiment showed that the total nitrogen content of nutrient solution in Brassica juncea seedlings was 9 mmol·L-1 (N3 treatment) with the highest leaf number, the largest leaf length and the largest leaf width at 14 d after transplanting, respectively Which was 4.03 cm and 3.00 respectively.The differences between the two strains and the N0 treatment were all significant at the level of 1, 2.43 cm and 1.08 cm. At 42 days after transplanting, the plant height, leaf number, maximum leaf length, shoots, underground parts, total biomass, the active root absorption area and the longest root length of B. juncea seedlings were 4.30 cm and 5.00 Results showed that there was a significant difference between them and N0 except for the difference between maximum leaf length and N0 treatment. The activities of CAT and POD in leaves of N3 treatment were higher, which were 16.89 U.mg-1.min-1 and 134.27 U.mg-1.min-1, respectively. The content of MDA was the lowest (2.29 nmol.g-1) The differences reached the extremely significant level. Therefore, under the conditions of this experiment, seedlings with the total nitrogen content of 9 mmol·L-1 (N3) in nutrient solution had the best growth and enzyme activities. It is suggested that the total nitrogen content in nutrient solution is 9 mmol.L-1.