目的建立高效液相色谱-串联质谱法同时测定豆芽中7种植物生长调节剂残留量的分析方法。方法豆芽样品经组织捣碎,用含1%乙酸-乙腈(1∶99,V/V)溶液提取,分散固相萃取吸附剂(C18粉末和无水硫酸镁)净化,溶剂转化定容后,采用Waters Acquity BEH C18色谱柱分离,电喷雾-负离子多反应监测模式(MRM)检测,基质匹配标准溶液外标法定量。结果当质量浓度范围为5μg/L~100μg/L时,各种植物生长调节剂的线性关系良好,相关系数均≥0.999 1,方法的检出限为0.5μg/kg~1.5μg/kg,方法的定量限为1.5μg/kg~5.0μg/kg,样品添加回收试验的平均回收率为80%~95%,相对标准偏差为3.9%~8.1%。结论该方法快速简便,方法的灵敏度高,定性、定量准确,可满足豆芽中7种植物生长调节剂残留量的检测要求。 OBJECTIVE To establish a method for simultaneous determination of 7 plant growth regulators in bean sprouts by high performance liquid chromatography-tandem mass spectrometry. Methods The bean sprouts samples were mashed by tissue and purified by solid-phase extraction adsorbent (C18 powder and anhydrous magnesium sulfate) with 1% acetic acid-acetonitrile (1:99, V / V) The separation was performed on a Waters Acquity BEH C18 column, electrospray ionization-anion multiple reaction monitoring (MRM) and matrix-matched standard external standard. Results When the concentration range of 5μg / L ~ 100μg / L, the linear relationship between various plant growth regulators was good, the correlation coefficient was ≥0.999 1, the detection limit was 0.5μg / kg ~ 1.5μg / kg, the method The limit of quantification was 1.5μg / kg ~ 5.0μg / kg. The average recoveries of samples were 80% ~ 95% and the relative standard deviations were 3.9% ~ 8.1%. Conclusion The method is rapid and simple, the method has high sensitivity, qualitative and quantitative accuracy, which can meet the detection requirements of 7 kinds of plant growth regulators in bean sprouts.