论文部分内容阅读
背景:近年来的研究表明,中药栀子可能具有活化软骨细胞的功能,而京尼平苷是栀子中重要成分之一。目的:观察京尼平苷对体外培养大鼠膝关节软骨细胞Ⅱ型胶原蛋白合成的影响。方法:体外分离培养大鼠膝关节软骨细胞,分别应用25,50及100 mmol/L京尼平苷干预,并设置正常对照组。RT-PCR和Western blot法观察京尼平苷对各组细胞Ⅱ型胶原mRNA和蛋白表达的影响。结果与结论:RT-PCR和Western blot法检测结果显示,与正常对照组相比,25,50及100 mmol/L京尼平苷干预可以明显促进Ⅱ型胶原mRNA及蛋白的表达(P<0.01)。结果证实,京尼平苷可以促进大鼠膝关节软骨细胞Ⅱ型胶原蛋白的合成。
BACKGROUND: Studies in recent years show that Chinese gardenia may have the function of activating chondrocytes, and geniposide is one of the important components in gardenia. Objective: To observe the effect of geniposide on the synthesis of type Ⅱ collagen in rat knee chondrocytes. Methods: Rat knee articular chondrocytes were isolated and cultured in vitro. Serum 25, 50 and 100 mmol / L of geniposide were used respectively to establish the normal control group. The effects of geniposide on the expression of type Ⅱ collagen mRNA and protein in each group were observed by RT-PCR and Western blot. RESULTS AND CONCLUSION: The results of RT-PCR and Western blot showed that geniposide at 25, 50 and 100 mmol / L could significantly promote the expression of type Ⅱ collagen mRNA and protein (P <0.01) compared with the normal control group ). The results confirmed that geniposide can promote type II collagen synthesis in rat knee chondrocytes.