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本文主要研究了油茶对高脂饮食引起的大鼠肝损伤的保护作用。试验中将大鼠分为空白对照组、高脂模型组、油茶低、中和高剂量组,每组10只。经造模期15 d和给样期32 d后,腹主动脉采血,取肝脏。测定大鼠肝脏重量,观察肝脏组织病理;分别测定血清中ALT和AST活力,血清和肝匀浆中SOD、GSH-Px活力和MDA水平。结果表明,与高脂模型组相比,油茶组肝脏系数显著降低(p<0.05);血清中ALT和AST活力显著降低(p<0.05);血清和肝脏中SOD和GSH-Px活力显著升高,MDA含量显著降低(p<0.05);肝脏组织病理显示,油茶组能够减轻肝细胞脂肪变性。由结果可知:油茶能够缓解高脂饮食大鼠肝脏肥大,增强机体抗脂质过氧化能力,缓解肝脏脂肪变性,对肝脏损伤起到有效的保护作用,其中油茶高剂量组作用最明显。
This paper mainly studied the protective effects of Camellia oleifera on liver injury induced by high-fat diet in rats. In the experiment, rats were divided into blank control group, high fat model group, low, medium and high dose of Camellia oleifera, 10 rats in each group. After modeling 15 d and sample period 32 d, abdominal aorta blood, take the liver. The liver weight and liver histopathology were measured. The activity of ALT and AST in serum and the activities of SOD, GSH-Px and MDA in serum and liver homogenate were measured respectively. The results showed that compared with the high-fat model group, the liver coefficient of Camellia oleifera decreased significantly (p <0.05), the activity of ALT and AST decreased significantly (p <0.05), the activities of SOD and GSH-Px in serum and liver increased significantly , MDA content was significantly lower (p <0.05); liver histopathology showed that the Camellia oleifera group can reduce fatty degeneration of liver cells. From the results we can see: Camellia can alleviate the liver hypertrophy of rats fed with high-fat diet, enhance the body’s ability to resist lipid peroxidation, relieve liver steatosis, liver injury play an effective protective effect, including the highest effect of the Camellia high dose group.