在人类肛殖癌症病因的研究中,最初人们把注意力集中于人类单纯疱疹病毒(HSV)。1974年以来,日益认识到人类乳头瘤病毒(HPV)的致病作用。实验室诊断 (一)分子杂交法病毒DNA的检测有多种方法:(1)Southern吸印法,可对不同HPV特有的DNA片段进行鉴定;(2)将放射标记的全部细胞DNA与不同型别的HPV克隆DNA杂交,称反向杂交;(3)点-吸印分析法。上述三种方法(特别是第一种方法)为高灵敏试验。改变杂交条件,使探针不仅与完全 In the study of the etiology of human anogenital cancers, initially people focused their attention on human herpes simplex virus (HSV). Since 1974, the pathogenic role of human papillomavirus (HPV) has been increasingly recognized. Laboratory Diagnosis (a) molecular hybridization virus DNA detection of a variety of methods: (1) Southern blotting method, different HPV-specific DNA fragments identified; (2) the radio-labeled cells with different types of DNA Other HPV cloning DNA hybridization, said reverse hybridization; (3) point - blot analysis. The above three methods (especially the first method) are highly sensitive tests. Change the hybridization conditions so that the probe is not only perfect