【摘 要】
：
目的 研究苹果对实验性肝纤维化大鼠的辅助治疗作用及疗效机理.方法 雄性SD大鼠42只分为正常组、CCL4肝纤维化模型组、自然恢复组、丹芍化纤治疗组、丹芍化纤+苹果试验组.除
【机 构】
：
贵阳医学院附属医院感染科,550004;
论文部分内容阅读
目的 研究苹果对实验性肝纤维化大鼠的辅助治疗作用及疗效机理.方法 雄性SD大鼠42只分为正常组、CCL4肝纤维化模型组、自然恢复组、丹芍化纤治疗组、丹芍化纤+苹果试验组.除正常组以外,其余大鼠采用CCL4[皮下注射40%CCL4+花生油溶液混合液0.3 mL/100 g首次用纯CCL4 0.5 mL/100 g,2次/周]、饮酒(隔日以30%乙醇为饮料)、高脂低蛋白饮食(79.5%玉米粉,20%猪油,0.5%胆固醇)等复合因素刺激制备肝纤维化动物模型共8周.8周末随机处死模型组大鼠8只,确认适模成功后将刺余模型组大鼠随机分为丹芍化纤[给予丹芍化纤粉末加蒸馏水混合溶液:药物浓度为0.1 g/100 g大鼠体质量配比,以0.2 mL/(10g·d)灌胃1次]+苹果试验组(新鲜苹果切碎组织匀浆机匀浆后过滤取汁为4 mL/100 g大鼠体质量灌胃,同时苹果渣做饲料喂养大鼠),丹芍化纤治疗组及自然恢复组(给予生理盐水等量灌胃),共观察8周.实验结束后光镜下观察肝组织纤维化程度,免疫组化SP法检测肝脏中COL-I,TIMP-1的表达.结果 免疫组化中丹芍化纤+苹果试验组肝组织中I型胶原(collagen-I,COL-I),基质金属蛋白酶组织押制因子1(Tissue inhibitor of metalloproteinase-1,TIMP-1)的表达与模型组、自然恢复组、丹芍化纤组比较显著降低(P＜0.01,P＜0.05).结论 苹果在实验性肝纤维化大鼠的治疗中起到了有效的作用,其抑制脂质过氧化作用在丹芍化纤的治疗基础上进一步抑制了TIMP-I、COL-I的表达;从而促进细胞外基质的降解,起到保护肝脏的作用,这可能是苹果辅助逆转肝纤维化的机制之一.
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