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迄今,人们仅能使用标记的甾体来定性或定量分析受体蛋白,这是当今甾体受体研究的主要局限性。近年,使用对抗雌激素,糖皮质激素、孕酮以及维生素 D_3等的受体的单克隆抗体,已为阐明上述受体的结构和功能提供了一种新型的工具。最近,有报告描述在人血清中存在某种对抗雄激素受体的自身抗体,作者在前列腺癌患者的血清中鉴定出高滴度的抗雄激素受体的自身抗体,并对其滴度、亲合力及特异性进行了分析。随后,作者从这些患者的血液中分离出淋巴细胞,经转化、克隆从而产生了对抗雄激素受体的单克隆抗体,并对其免疫学特性进行了研究。从多种来源得组织(如肾上腺切除大鼠的肝脏,阉割24小时 SD 大鼠的腹侧前列腺,精囊,完整 SD 大鼠的睾丸、附睾以及23天大小的雌激素处
Hitherto, one can only characterize or quantify receptor proteins using labeled steroids, which is a major limitation of today’s research on steroid receptors. In recent years, the use of monoclonal antibodies against estrogens, glucocorticoids, progesterone, and vitamin D3 receptors has provided a new tool to elucidate the structure and function of these receptors. Recently, there have been reports describing the presence of some autoantibody against human androgen receptor in human serum. The authors identified high titers of anti-androgen receptor autoantibodies in the serum of prostate cancer patients and determined their titer, Affinity and specificity were analyzed. Subsequently, the authors isolated lymphocytes from the blood of these patients, transformed and cloned to produce monoclonal antibodies against the androgen receptor, and studied their immunological properties. Tissues from a variety of sources, such as the liver of adrenalectomized rats, were castrated 24 hours in the ventral prostate of SD rats, seminal vesicle, testis of intact SD rats, epididymis and 23 days of estrogen