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MYB类转录因子是植物转录因子最大的家族之一,参与控制植物腺毛细胞的模式和形态建成。本研究利用雷蒙德氏棉(Gossypium raimondii)D5基因组数据库以AtMYB0(GL1,NM_113708)蛋白为参比序列获得同源基因GrMYB0,从徐州142中克隆了陆地棉的GhMYB0,其开放阅读框长度为843 bp,编码280个氨基酸。经过保守结构域分析和亚细胞定位确定GhMYB0为R2R3-MYB转录因子。qRT-PCR的结果表明,GhMYB0在徐州142开花当天开始高调表达,开花后20 d表达量达高峰;在所有的组织器官中,花中表达量最高,其次为胚珠。转基因功能分析结果表明,在野生型拟南芥(Columbia)中过表达GhMYB0,使其叶片表皮毛与野生型相比明显减少;该基因在拟南芥突变体gl-1中过表达,能恢复表皮毛缺失型突变体的表型,说明该基因可能对拟南芥表皮毛的形态建成发挥一定作用,本试验为研究R2R3-MYB转录因子在棉纤维起始和伸长过程中的调控作用提供有力证据。
MYB transcription factor is one of the largest families of plant transcription factors involved in the control of plant hair cell morphology and morphogenesis. In this study, the homologous gene GrMYB0 was obtained by using the AtMYB0 (GL1, NM_113708) protein as reference sequence in Gossypium raimondii D5 genome database. The open reading frame of GhMYB0 was cloned from Xuzhou 142 with the length of 843 bp, encoding 280 amino acids. GhMYB0 was identified as R2R3-MYB transcription factor by conserved domain analysis and subcellular localization. The results of qRT-PCR showed that GhMYB0 began to express high-level on the day of flowering in Xuzhou 142 and peaked on the 20th day after flowering. The expression of GhMYB0 was the highest in all tissues and organs, followed by the ovules. The results of transgenic function analysis showed that overexpression of GhMYB0 in wild-type Arabidopsis resulted in a significant reduction of leaf epidermal hairs compared with that of wild-type; overexpression of this gene in Arabidopsis thaliana mutant gl-1 restored Epidermal hair loss mutant phenotype, indicating that the gene may play an important role in the formation of Arabidopsis epidermal hair. In this study, R2R3-MYB transcription factor to study the regulation of cotton fiber during the initiation and elongation of the force evidence.