利用基因枪技术 ,将小麦土传花叶病毒外壳蛋白基因CWMV CP1和筛选基因bar导入扬麦 15 8,获得14 5株抗Bialaphos再生植株 ;PCR Southern分析 ,其中 2 1株为阳性植株 ,转化率达到 0 .99% ;T1代植株的PCR Southern、单酶切和双酶切Southern杂交 ,证明外源抗性基因已经完整地整合到小麦基因组中 ;T1代植株分离比CP1+ ∶CP1-为 1∶1.3,偏离孟德尔分离定律 ;T2 代植株总RNA ,RT PCR的试验结果表明CWMV CP1在转录水平上得到了表达 Using gene gun technology, the wheat soil-derived mosaic virus gene CWMV CP1 and the screening gene bar were introduced into Yangmai 15 8 to obtain 145 resistant Bialaphos regenerated plants. PCR Southern analysis showed that 21 were positive plants and the transformation rate Reached0.99%. PCR Southern, Southern and Southern hybridization showed that the exogenous resistance genes had been integrated into the genome of wheat. The T1 generation plants were more stable than CP1 +: CP1- 1.3, deviation from Mendelian law of separation; T2 generation plant total RNA, RT PCR test results showed that CWMV CP1 was expressed at the transcriptional level