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为探讨河南省献血员中庚型肝炎病毒（ＨＧＶ）的感染情况和病毒基因的变异特征。方法：利 用逆转录－巢式－聚合酶链反应（ＲＴ－Ｎｅｓｔ－ＰＣＲ）检测 ＨＧＶ ＲＮＡ；克隆人 Ｔ载体并测序。结果：在 ３００名义务献血员 中发现１例庚型肝炎病毒ＲＮＡ阳性；２００例初检合格献血员标本中无１例阳性。河南株ＨＧＶ与ＧｅｎＢａｎｋ中ＨＧＶ 对应位置序列的同源性为９０.９％～９８．８％。结论：河南省义务献血员中有ＨＧＶ携带者；河南株ＨＧＶ与国内（河 北株ＨＧＵ７５３５６）分离株的同源性为９８．８％，其同源性高于国外分离株（９０．９％～９５．２％）；建议对献血员增加 ＨＧＶ 检测。
To investigate the hepatitis G virus (HGV) infection and the variation characteristics of the viral genes among blood donors in Henan Province. Methods: HGV RNA was detected by reverse transcription-nested-polymerase chain reaction (RT-PCR); human T vector was cloned and sequenced. Results: One case of hepatitis G virus RNA was found positive among 300 volunteer blood donors. None of the 200 first-pass donors had positive results. The homology between HGV in Henan and HGV in GenBank ranged from 90.9% to 98.8%. Conclusion: HGV carriers were found among voluntary blood donors in Henan Province. The homology of Henan HGV isolates with domestic isolates (Hebei HGU75356) was 98.8%, higher than that of foreign isolates (90.9% 95.2%); HGV testing is recommended for blood donors.