随着泳动距离增加而丙烯酰胺浓度也逐渐增加的梯度聚丙烯酰胺凝胶,目前已广泛用来代替单一浓度的凝胶。浓度梯度胶随浓度增加而孔径逐渐变小,这将对蛋白质组成的分析更为细致,同时能获得更清晰的蛋白质区带。现将我们用梯度胶和单一浓度胶对同一蛋白质组分进行比较的实验报告如下。 Gradient polyacrylamide gels with increasing concentrations of acrylamide as the distance of migration increases are now widely used instead of single concentrations of gels. The concentration gradient of the gel gradually increases with increasing pore size, which will provide a more detailed analysis of the protein composition and clearer protein bands. Now we use a gradient of gel and a single concentration of glue on the same protein composition of the experimental report is as follows.