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目的:观察软脉灵口服液对实验性血管性痴呆(VaD)大鼠空间学习记忆能力和自由基代谢的影响。方法:选用永久性结扎双侧颈总动脉(2VO)制作VaD模型,用Morris水迷宫测定大鼠学习记忆能力,比色法大鼠海马组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果:与模型组比较,软脉灵高剂量组逃避潜伏期缩短(P<0.001)、1min穿越原平台次数增加(P<0.001)、海马组织中SOD活性及GSH含量增加(P<0.001,P<0.01)、MDA含量下降(P<0.001);软脉灵低剂量组大鼠海马组织中SOD活性比模型组增加(P<0.01)、MDA含量下降(P<0.05);与软脉灵低剂量组比较,高剂量组大鼠海马组织中GSH含量更高(P<0.05),MDA含量更低(P<0.05)。结论:软脉灵口服液能改善拟VaD大鼠的空间学习记忆能力,减少拟VaD大鼠海马氧自由基堆积。这些作用呈一定的量效关系。
Objective: To observe the effect of Ruanmailing Oral Liquid on spatial learning and memory ability and free radical metabolism in experimental vascular dementia (VaD) rats. METHODS: The VaD model was established by permanently ligating bilateral common carotid arteries (2VO). The learning and memory abilities of rats were determined by Morris water maze. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in the hippocampus of rats were determined by colorimetry. MDA) and reduced glutathione (GSH) content. RESULTS: Compared with the model group, the escape latency of the high dose group of Ruanmailing Ling was shortened (P<0.001), the number of crossing the original platform 1min (P<0.001), the activity of SOD and the content of GSH in the hippocampus increased (P<0.001, P< 0.01), MDA content decreased (P <0.001); SOM activity in hippocampus of rats in the low dose group of Ruan Mai Ling increased (P <0.01), MDA content decreased (P <0.05); In group comparisons, the GSH content in the hippocampus of rats in the high-dose group was higher (P<0.05) and the MDA content was lower (P<0.05). Conclusion: Ruanmailing Oral Liquid can improve the spatial learning and memory ability of VaD rats and reduce the accumulation of oxygen free radicals in hippocampus of VaD rats. These effects have a certain dose-effect relationship.